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试剂耗材

丝裂霉素 C ,Mitomycin C from Streptomyces caespitosus powder, contains NaCl as solubilizer

产品介绍【返回】

品牌: sigma
货号: M0503
CAS号: 50-07-7
供应商: 上海觅拓
数量: 大量
规格: 2MG

Application

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.

Biochem/physiol Actions

DNA 合成、细胞核分裂和癌细胞的抑制剂。对革兰氏阳性菌、革兰氏阴性菌、抗酸杆菌有抗菌性。

Caution

This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared - the precipitated solution has been proven toxic to cells.

Preparation Note

Mitomycin C is soluble in water at 。5 mg/mL, with a pH of 6。0-7。5。 It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9。

Protocols & Applications

性质

Related Categories , , ,, ,
form   powder
contains   NaCl as solubilizer
solubility   H2O: soluble1 vial + 4 mL, clear to slightly hazy, blue to purple
Mode of action   DNA synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
storage temp.   2-8°C
  • C15H18N4O5

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